St Nicholas Orthodox Church, DuBois, PA began as a mission in 1978 under the spiritual guidance of His Eminence, Archbishop Job of Chicago, who was then Rector of St John the Baptist Church, Black Lick, PA. Services were held in various churches and buildings by Deanery clergy until the parish found its present home. Through the hard labor of this small group, generous gifts and loans from the Archdiocese of Western Pennsylvania (OCA), sister parishes, and pious individuals, St Nicholas Orthodox Mission finally had a home on North Third Street in 1980, along with a full-time parish priest.

In April of 2020, with the assistance of the Archdiocese, the parishioners of St Nicholas purchased the former St Joseph Roman Catholic Church on South Street. The new location is fully ADA accessible with a large social hall for the several events the parish conducts each year. Renovations are ongoing to transform the building into beautiful, Orthodox-styled church. Pardon our dust! (The original location remains as an icon studio and home for Fr Mark Meholick, our rector, as well as a chapel for select services.)

The parish is combined administratively with Saints Peter and Paul Church, Urey, PA, which is about a 45-minute drive from DuBois. Until 2021, both parishes were served by Fr Mark, so the liturgical schedule was shared between the two churches. After the ordination of Fr Alexander Cadman in September of 2021, however, the Divine Liturgy is now celebrated every Sunday at St Nicholas. Glory to Jesus Christ!